Occurrence and potentially zoonotic genotypes of Enterocytozoon bieneusi in wild rhesus macaques (Macaca mulatta) living in Nanwan Monkey Island, Hainan, China: a public health concern

Abstract Background Enterocytozoon bieneusi , a microsporidian species, is zoonotic pathogen found in both humans and animals. Here, we determined the prevalence, explored different genotypes of E. wild rhesus macaques ( Macaca mulatta ) (Hainan Island China), assessed their potential. Methods We collected 173 fecal specimens from living Nanwan Monkey Island, Hainan, China. Subsequently, identified genotyped using nested PCR analysis amplification internal transcribed spacer region (ITS) rRNA gene. Lastly, neighbor-joining tree was built based on gene sequences ITS E . Results Of macaques, 26 (15%) were infected with six which five known: PigEBITS7 n = 20), D 2), Type IV 1), Peru6 (n Henan-III novel genotype: HNM-IX 1). From phylogenetic analysis, here all clustered into group 1. Conclusion This study first report to detect infection Human-pathogenic D, Henan-III, Peru6, PigEbITS7, support these animals have public health significance.